Takeo T, Ogura M, Okumura M, Morishima Y, Hirabayashi N, Kitoh J, Saito H
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Leukemia. 1993 Aug;7(8):1268-74.
A human megakaryoblastic cell line (MEG-01) was successfully transplanted into athymic nude mice. MEG-01 cells (5 x 10(7)) were inoculated subcutaneously in KSN-nu/nu mice, none of which were pretreated with irradiation or chemotherapeutic agents. All mice developed solid tumors at the site of injection after incubation for 10-14 days reaching a size of 200-400 mm2 (product of cross-sectional diameters) after 30 days. These tumors, designated as MEG-01/nu, were transplanted into other nude mice. The transplanted tumors infiltrated the liver and spleen, and leukemic megakaryoblastic cells appeared in the blood of some transplanted mice. Cells resuspended from MEG-01/nu tumors exhibited almost the same megakaryocytic characteristics as the original MEG-01 cells, and underwent in vitro differentiation to a mature form of megakaryocyte upon addition of phorbol diesters. MEG-01/nu was evaluated for sensitivity to cytosine arabinoside, vincristine, and daunorubicin in vitro and in vivo. Daunorubicin exhibited significant anti-tumor activity against MEG-01/nu in vivo, while cytosine arabinoside did so in vitro. Vincristine showed no activity against these cells. This cell line may provide a useful model for testing the in vivo efficacy of anti-tumor agents and immunotoxins, and for studying the pathophysiological mechanisms of human megakaryoblastic leukemia.
一种人巨核母细胞系（MEG-01）成功移植到无胸腺裸鼠体内。将MEG-01细胞（5×10⁷个）皮下接种到KSN-nu/nu小鼠体内，这些小鼠均未接受过照射或化疗药物预处理。孵育10 - 14天后，所有小鼠在注射部位均形成实体瘤，30天后肿瘤大小达到200 - 400平方毫米（横径乘积）。这些肿瘤命名为MEG-01/nu，并移植到其他裸鼠体内。移植的肿瘤浸润肝脏和脾脏，部分移植小鼠的血液中出现白血病性巨核母细胞。从MEG-01/nu肿瘤中重悬的细胞表现出与原始MEG-01细胞几乎相同的巨核细胞特征，加入佛波酯后可在体外分化为成熟形式的巨核细胞。对MEG-01/nu进行了阿糖胞苷、长春新碱和柔红霉素的体内外敏感性评估。柔红霉素在体内对MEG-01/nu表现出显著的抗肿瘤活性，而阿糖胞苷在体外有此作用。长春新碱对这些细胞无活性。该细胞系可为测试抗肿瘤药物和免疫毒素的体内疗效以及研究人巨核母细胞白血病的病理生理机制提供有用的模型。
