Lu M H, Hinson W G, Turturro A, Sheldon W G, Hart R W
Food and Drug Administration, National Center for Toxicological Research (NCTR), Jefferson, Arkansas 72079.
Mech Ageing Dev. 1993 May;68(1-3):151-62. doi: 10.1016/0047-6374(93)90147-j.
The effect of dietary restriction (DR) on cell proliferation determined by cell cycle analysis in tissues of young and old mice was investigated. Using the percentage of S-phase cells as an index of cell proliferation, we found that DR inhibited cell proliferation in spleen and thymus in young mice. No significant changes were found in bone marrow and kidney in the ad libitum (AL) or DR mice regardless of age. In old mice, the DR effect was observed in spleen only. When age increased, a parallel decline in cell proliferation was evidenced by a reduced % of S-phase cells. DR produces a greater cell cycle effect in the young mice than in the old mice. The present data suggests that inhibition of cell proliferation by DR may be affected by type of tissue, age, length of DR, and capacity or rate of cell proliferation.
研究了饮食限制（DR）对通过细胞周期分析测定的年轻和老年小鼠组织中细胞增殖的影响。以S期细胞百分比作为细胞增殖指标，我们发现DR抑制了年轻小鼠脾脏和胸腺中的细胞增殖。无论年龄大小，自由采食（AL）或DR小鼠的骨髓和肾脏均未发现显著变化。在老年小鼠中，仅在脾脏中观察到DR效应。随着年龄增长，S期细胞百分比降低证明细胞增殖呈平行下降。DR对年轻小鼠产生的细胞周期效应比老年小鼠更大。目前的数据表明，DR对细胞增殖的抑制可能受组织类型、年龄、DR时长以及细胞增殖能力或速率的影响。
