Intraneoplastic injection of CCNU for experimental brain tumor chemotherapy.

Chemotherapy experiments were performed with 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) in an experimental mouse brain tumor model. Cell suspensions of a transplantable mouse ependymoblastoma were injected intracerebrally by means of a stereotactic frame. CCNU was given either intraperitoneally or by direct intraneoplastic injection beginning the fifth day after tumor cell implantation. Intraneoplastic injection beginning the fifth day after tumor cell implantation. Intraneoplastic injections of drugs were made with the stereotactic frame. A single intraneoplastic injection of CCNU was found to be highly effective in increasing the median day of death and in yielding large numbers of long-term survivors. In some experiments CCNU injected into the neoplasm produced increased numbers of long-term survivors and less systemic toxicity than when injected intraperitoneally.