Nerurkar A V, Vaidya N R, Advani S H, Gothoskar B P
Division of Biological Chemistry, Tata Memorial Center, Parel, Bombay, India.
Neoplasma. 1993;40(1):31-4.
Ferritin, an iron-storing protein, was isolated from disease-involved and -uninvolved regions of spleen biopsies obtained from patients with Hodgkin's disease (HD). Ferritin from all human spleen biopsies showed a major band of polypeptide of M(r) around 20 kDa in 1D-SDS-PAGE analysis. The corresponding bands for horse spleen ferritin and apoferritin (Sigma) were at a slightly lower M(r) level. In isoelectrofocusing (IEF) studies, the pI values of human spleen ferritin from the uninvolved and involved regions were 4.55 and 4.14, respectively. These were more acidic than that of horse spleen ferritin (4.79). Human spleen ferritin from the involved region also differed from that of the uninvolved region in the pattern of CNBr-generated peptide maps in 1D-SDS-PAGE. These results suggest that the presence of Hodgkin's disease in human spleen is associated with some physiochemical changes in the tissue ferritin.
铁蛋白是一种铁储存蛋白，从霍奇金淋巴瘤（HD）患者的脾脏活检的病变和未病变区域中分离得到。在一维SDS-PAGE分析中，所有人类脾脏活检样本中的铁蛋白均显示出一条主要的多肽带，其相对分子质量（M(r)）约为20 kDa。马脾铁蛋白和脱铁铁蛋白（Sigma）的相应条带的M(r)水平略低。在等电聚焦（IEF）研究中，来自未病变和病变区域的人类脾脏铁蛋白的等电点（pI）值分别为4.55和4.14。这些值比马脾铁蛋白的等电点（4.79）更偏酸性。在一维SDS-PAGE中，来自病变区域的人类脾脏铁蛋白在溴化氰生成的肽图谱模式上也与未病变区域的不同。这些结果表明，人类脾脏中霍奇金淋巴瘤的存在与组织铁蛋白的一些理化变化有关。
