Shaw C, Cormican K, Thim L, Maule A G, Sloan J M, Buchanan K D
Department of Medicine, Queen's University of Belfast, UK.
Regul Pept. 1993 Jun 11;45(3):387-94. doi: 10.1016/0167-0115(93)90365-f.
Using an antiserum raised to the C-terminal region of neuropeptide Y (NPY) which does not cross-react with pancreatic polypeptide (PP), immunoreactivity has been detected in two different endocrine tumours of the human pancreas in concentrations permitting isolation and structural analysis. In a clinically-typical gastrinoma, resected from the head of pancreas, the concentration of NPY immunoreactivity was 3.4 nmol/g. Reverse phase HPLC analysis of extracts of this tumour resolved a single immunoreactive peptide coeluting with synthetic human NPY. The molecular mass of the isolated peptide, determined by mass spectroscopy, was 4270 Da, which was in close agreement with that derived from the deduced primary structure of human tumour NPY (4271.7 Da), obtained by gas-phase sequencing. A somatostatinoma, resected from the region of the ampulla of Vater, contained 3.8 nmol/g of NPY immunoreactivity and isolation of this immunoreactive peptide followed by structural analyses, indicated a molecular structure consistent with NPY 3-36. These data suggest that NPY immunoreactivity detected in human pancreatic endocrine tumours is molecularly heterogenous, a finding which may be of relevance in the symptomatology of such tumours as attenuation of the N-terminus of this peptide generates receptor selectivity.
使用一种针对神经肽Y（NPY）C末端区域产生的抗血清，该抗血清与胰多肽（PP）无交叉反应，在人类胰腺的两种不同内分泌肿瘤中检测到了免疫反应性，其浓度允许进行分离和结构分析。在从胰头切除的临床典型胃泌素瘤中，NPY免疫反应性浓度为3.4 nmol/g。对该肿瘤提取物进行反相高效液相色谱分析，分离出一种与合成人NPY共洗脱的单一免疫反应性肽。通过质谱法测定，分离出的肽的分子量为4270 Da，这与通过气相测序获得的人肿瘤NPY推导一级结构（4271.7 Da）得出的分子量非常一致。从 Vater壶腹区域切除的一个生长抑素瘤，含有3.8 nmol/g的NPY免疫反应性，对这种免疫反应性肽进行分离并随后进行结构分析，表明其分子结构与NPY 3-36一致。这些数据表明，在人类胰腺内分泌肿瘤中检测到的NPY免疫反应性在分子上是异质的，这一发现可能与此类肿瘤的症状学相关，因为该肽N末端的衰减会产生受体选择性。
