高效液相色谱法与²⁵²Cf等离子体解吸质谱法联用鉴定人参酊剂的成分

Combination of HPLC and 252-Cf plasma desorption mass spectrometry for identifying composition of ginseng tinctures.

作者信息

Elkin Y N, Makhankov V V, Uvarova N L, Bondarenko P V, Zubarev R A, Knysh A N

机构信息

Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, Vladivostok.

出版信息

Zhongguo Yao Li Xue Bao. 1993 Mar;14(2):97-100.

PMID:8352021

Abstract

The 252-Cf plasma desorption mass spectrometry (252-Cf PDMS) determination or confirmation of the ginsenoside saponins has been proposed to investigate the composition of high performance liquid chromatography (HPLC) peaks of ginseng tinctures and galenic preparations. That ionization technique is well suitable for the analysis of natural mixtures of these saponins. The 252-Cf PD mass spectra of standard ginsenosides Rb1, Rb2, Rc, Re, Rg1, Rd, NG-R2, Z-R1 contain the peaks of two types of ions, namely, molecular adduct ions (MAI) and aglycone ions. By mass the latter may be referred to either protopanaxadiol or protopanaxatriol. The masses of MAI and aglycone ions are determined by the carbohydrate chains. The collected HPLC fractions of P ginseng tincture can be tested for content of ginsenosides. After studying two MAI peaks from the 252-Cf PD mass spectra of the basic ginsenosides, an example of distinction between two galenic preparations from different Panax has been shown.

摘要

有人提出用²⁵²Cf等离子体解吸质谱法（²⁵²Cf PDMS）测定或确证人参皂苷，以研究人参酊剂和盖伦制剂高效液相色谱（HPLC）峰的组成。该电离技术非常适合分析这些皂苷的天然混合物。标准人参皂苷Rb1、Rb2、Rc、Re、Rg1、Rd、NG-R2、Z-R1的²⁵²Cf PD质谱包含两种离子峰，即分子加合离子（MAI）和苷元离子。从质量上看，后者可能指原人参二醇或原人参三醇。MAI和苷元离子的质量由碳水化合物链决定。收集的人参酊剂HPLC馏分可检测人参皂苷含量。在研究了基本人参皂苷²⁵²Cf PD质谱中的两个MAI峰后，展示了区分不同人参属植物两种盖伦制剂的一个实例。