Feelders R A, Vreugdenhil G, van Dijk J P, Swaak A J, van Eijk H G
Department of Chemical Pathology, Erasmus University, Rotterdam, The Netherlands.
Am J Hematol. 1993 Jul;43(3):200-4. doi: 10.1002/ajh.2830430308.
In anemia of chronic disease (ACD) in rheumatoid arthritis (RA) a decreased iron uptake and transferrin binding by erythroblasts are postulated to play a pathophysiological role. To examine whether this is related to changes in transferrin receptor expression by erythroblasts, we studied bone marrow from 5 healthy controls, 5 nonanemic RA patients, and 9 RA patients with ACD. Bone marrow mononuclear cells were incubated with increasing concentrations of 125I-transferrin and specific binding data were analyzed by the method of Scatchard. The number of transferrin receptors on erythroblasts from RA patients with ACD was significantly lower as compared to nonanemic RA patients (P < .05) and controls (P < .02). The affinity of the transferrin receptor tended to be lower in ACD. These preliminary data may indicate that transferrin receptor expression by erythroblasts is impaired in ACD. Since the rate of erythroid iron uptake is mainly determined by the number of transferrin binding sites, this may explain a decrease in erythroblast iron availability in ACD in RA.
在类风湿关节炎（RA）所致的慢性病贫血（ACD）中，推测成红细胞铁摄取减少及转铁蛋白结合减少在病理生理过程中起作用。为了研究这是否与成红细胞转铁蛋白受体表达的变化有关，我们研究了5名健康对照者、5名非贫血RA患者及9名患有ACD的RA患者的骨髓。将骨髓单个核细胞与浓度递增的125I-转铁蛋白一起孵育，并采用Scatchard法分析特异性结合数据。与非贫血RA患者（P < 0.05）及对照者（P < 0.02）相比，患有ACD的RA患者成红细胞上的转铁蛋白受体数量显著减少。在ACD中，转铁蛋白受体的亲和力有降低趋势。这些初步数据可能表明，ACD中，成红细胞的转铁蛋白受体表达受损。由于红系铁摄取率主要由转铁蛋白结合位点数量决定，这可能解释了RA患者ACD中，成红细胞铁利用率降低的原因。
