Kenmochi N, Maeda N, Tanaka T
Department of Biochemistry, School of Medicine, University of the Ryukyus, Okinawa, Japan.
Biochem Biophys Res Commun. 1993 Aug 16;194(3):1131-4. doi: 10.1006/bbrc.1993.1939.
We have examined the binding of chicken nuclear protein to the promoter regions of chicken ribosomal protein L5 and 5S rRNA genes. A nuclear protein was shown to bind to similar sequences in both promoter regions of the L5 gene (-36 to -21) and the internal control region of 5S rRNA gene (56 to 71). Its molecular mass was estimated to be 34 kDa. Competition gel mobility assay showed that the protein is different from the TATA-box binding protein (TBP). The protein may play a role in a transcriptional coordination of the two genes transcribed by different polymerases, RNA polymerase II and III.
我们检测了鸡核蛋白与鸡核糖体蛋白L5基因和5S rRNA基因启动子区域的结合情况。结果显示，一种核蛋白可与L5基因启动子区域（-36至-21）和5S rRNA基因内部控制区域（56至71）中的相似序列结合。其分子量估计为34 kDa。竞争凝胶迁移率分析表明，该蛋白与TATA框结合蛋白（TBP）不同。该蛋白可能在由不同聚合酶（RNA聚合酶II和III）转录的这两个基因的转录协调中发挥作用。
