Frederiksen Sune, Heeno Andersen Jesper
Department of Medical Biochemistry and Genetics, Biochemistry Laboratory B, The Panum Institute, University of Copenhagen, Copenhagen, Denmark.
Hereditas. 2003;139(2):156-60. doi: 10.1111/j.1601-5223.2003.01796.x.
The guinea pig has about 100 copies of the 5S rRNA gene per haploid genome and they are present in 2.1 kb tandem repeats. Three bona fide 5S rRNA genes and four pseudo genes were sequenced. The conserved external promoter (D box) found in rodents and primates is only partially present in the guinea pig. The "D box like" sequence in guinea pig only has eight of the 12 nucleotides in the conserved D box. The results are in accordance with investigations showing that the guinea pig is not a rodent. Conserved sequences in the non-transcribed spacer can therefore be useful in phylogenetic studies.
豚鼠单倍体基因组中约有100个5S rRNA基因拷贝，它们以2.1 kb的串联重复序列形式存在。对三个真正的5S rRNA基因和四个假基因进行了测序。在啮齿动物和灵长类动物中发现的保守外部启动子（D框）在豚鼠中仅部分存在。豚鼠中的“类D框”序列在保守的D框的12个核苷酸中只有8个。这些结果与表明豚鼠不是啮齿动物的研究一致。因此，非转录间隔区中的保守序列可用于系统发育研究。
