从雌性家蝇（Musca domestica）附腺中分离并鉴定一种新型肽。

Isolation and identification of a novel peptide from the accessory sex gland of the female house fly, Musca domestica.

作者信息

Wagner R M, Woods C W, Hayes J A, Kochansky J P, Hill J C, Fraser B A

机构信息

United States Department of Agriculture, Livestock Insects Laboratory, Livestock, Beltsville, MD.

出版信息

Biochem Biophys Res Commun. 1993 Aug 16;194(3):1336-43. doi: 10.1006/bbrc.1993.1971.

DOI:10.1006/bbrc.1993.1971

PMID:8352792

Abstract

A peptide of novel structure was isolated from accessory glands of the female house fly, Musca domestica by molecular-weight limit centrifugal ultrafiltration and reversed-phase HPLC. After amino acid and protein sequence analyses, the structure of this peptide was confirmed by chemical synthesis and plasma desorption mass spectral analysis to be Leu-Leu-Asn-Ala-Leu-Pro-Leu-Asp-Ala-Leu-Ser-Ser-Leu-Thr-Gly-NH2. The accessory gland peptide stimulated contraction of the house fly oviduct at concentrations of 10(-7) M and above.

摘要

通过分子量限制离心超滤和反相高效液相色谱法，从雌性家蝇（Musca domestica）的副腺中分离出一种结构新颖的肽。经过氨基酸和蛋白质序列分析后，通过化学合成和等离子体解吸质谱分析确定该肽的结构为Leu-Leu-Asn-Ala-Leu-Pro-Leu-Asp-Ala-Leu-Ser-Ser-Leu-Thr-Gly-NH2。该副腺肽在浓度为10^(-7) M及以上时刺激家蝇输卵管收缩。