Homayoun P, Stacey D W
Department of Molecular Biology, Cleveland Clinic Foundation, OH 44106.
Biochem Biophys Res Commun. 1993 Aug 16;194(3):1413-9. doi: 10.1006/bbrc.1993.1982.
The intrinsic GTPase activity of cellular protein p21ras is strongly increased by cytosolic GTPase activating protein (GAP). The activity of this enzyme has been shown to be inhibited by arachidonic acid. We report here that this inhibition is antagonized by the presence of 1-stearoyl, 2-arachidonoyl glycerol. This effect is structure specific and dose dependent, being maximum at 200 micrograms/ml of diacylglycerol (DG). These results suggest that production of DG in response to hormones or growth factors stimulation could indirectly modulate the interaction between p21ras and GAP.
胞质GTP酶激活蛋白（GAP）可显著增强细胞蛋白p21ras的内在GTP酶活性。该酶的活性已被证明会受到花生四烯酸的抑制。我们在此报告，1-硬脂酰-2-花生四烯酰甘油的存在可拮抗这种抑制作用。这种效应具有结构特异性且呈剂量依赖性，在二酰基甘油（DG）浓度为200微克/毫升时达到最大值。这些结果表明，响应激素或生长因子刺激而产生的DG可能间接调节p21ras与GAP之间的相互作用。
