花生四烯酸对GTP酶激活蛋白的抑制作用被1-硬脂酰-2-花生四烯酸甘油酯拮抗。

Inhibitory effect of arachidonic acid on GTPase activating protein is antagonized by 1-stearoyl, 2-arachidonoyl glycerol.

作者信息

Homayoun P, Stacey D W

机构信息

Department of Molecular Biology, Cleveland Clinic Foundation, OH 44106.

出版信息

Biochem Biophys Res Commun. 1993 Aug 31;195(1):144-50. doi: 10.1006/bbrc.1993.2022.

DOI:10.1006/bbrc.1993.2022

PMID:8363595

Abstract

The intrinsic GTPase activity of cellular protein p21ras is strongly increased by cytosolic GTPase activating protein (GAP). The activity of this enzyme has been shown to be inhibited by arachidonic acid. We report here that this inhibition is antagonized by the presence of 1-stearoyl, 2-arachidonoyl glycerol. This effect is structure specific and dose dependent, being maximum at 200 micrograms/ml of diacylglycerol (DG). These results suggest that production of DG in response to hormones or growth factors stimulation could indirectly modulate the interaction between p21ras and GAP.

摘要

细胞蛋白p21ras的内在GTP酶活性被胞质GTP酶激活蛋白（GAP）强烈增强。该酶的活性已被证明受花生四烯酸抑制。我们在此报告，1-硬脂酰-2-花生四烯酰甘油的存在可拮抗这种抑制作用。这种效应具有结构特异性且呈剂量依赖性，在二酰基甘油（DG）浓度为200微克/毫升时达到最大值。这些结果表明，响应激素或生长因子刺激而产生的DG可能间接调节p21ras与GAP之间的相互作用。