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精浆核糖核酸酶二聚体结构的决定因素位于其N端区域。

The determinants of the dimeric structure of seminal ribonuclease are located in its N-terminal region.

作者信息

Di Donato A, Cafaro V, de Nigris M, Rizzo M, D'Alessio G

机构信息

Dipartimento di Chimica Organica e Biologica, Università di Napoli Federico II, Italy.

出版信息

Biochem Biophys Res Commun. 1993 Aug 16;194(3):1440-5. doi: 10.1006/bbrc.1993.1986.

DOI:10.1006/bbrc.1993.1986
PMID:8352802
Abstract

A chimeric cDNA was constructed, coding for the N-terminal region of BS-RNase (residues 1-49) and the C-terminal region of RNase A (residues 50-124). The resulting chimeric DNA was expressed in Escherichia coli and found to code for RNase chains that spontaneously assembled into a covalently dimeric ribonuclease.

摘要

构建了一个嵌合cDNA，其编码BS-RNase的N端区域（第1-49位氨基酸残基）和RNase A的C端区域（第50-124位氨基酸残基）。所得的嵌合DNA在大肠杆菌中表达，发现其编码的RNase链能自发组装成共价二聚体核糖核酸酶。

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The determinants of the dimeric structure of seminal ribonuclease are located in its N-terminal region.精浆核糖核酸酶二聚体结构的决定因素位于其N端区域。
Biochem Biophys Res Commun. 1993 Aug 16;194(3):1440-5. doi: 10.1006/bbrc.1993.1986.
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Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase.铰链肽和亚基间界面在二聚体牛精浆核糖核酸酶N端交换中的作用
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The swapping of terminal arms in ribonucleases: comparison of the solution structure of monomeric bovine seminal and pancreatic ribonucleases.核糖核酸酶中末端臂的交换：单体牛精浆核糖核酸酶和胰核糖核酸酶溶液结构的比较
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Full antitumor action of recombinant seminal ribonuclease depends on the removal of its N-terminal methionine.重组精液核糖核酸酶的完全抗肿瘤作用取决于其N端甲硫氨酸的去除。
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引用本文的文献

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Molecular evolution of B6 enzymes: binding of pyridoxal-5'-phosphate and Lys41Arg substitution turn ribonuclease A into a model B6 protoenzyme.B6 酶的分子进化：磷酸吡哆醛的结合及赖氨酸 41 被精氨酸取代使核糖核酸酶 A 转变为一种 B6 原酶模型。
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The C-terminal region of human angiogenin has a dual role in enzymatic activity.人血管生成素的C末端区域在酶活性中具有双重作用。
Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3243-7. doi: 10.1073/pnas.93.8.3243.
3
Hints on the evolutionary design of a dimeric RNase with special bioactions.
具有特殊生物活性的二聚体核糖核酸酶的进化设计提示
Protein Sci. 1995 Aug;4(8):1470-7. doi: 10.1002/pro.5560040804.