精浆核糖核酸酶二聚体结构的决定因素位于其N端区域。
The determinants of the dimeric structure of seminal ribonuclease are located in its N-terminal region.
作者信息
Di Donato A, Cafaro V, de Nigris M, Rizzo M, D'Alessio G
机构信息
Dipartimento di Chimica Organica e Biologica, Università di Napoli Federico II, Italy.
出版信息
Biochem Biophys Res Commun. 1993 Aug 16;194(3):1440-5. doi: 10.1006/bbrc.1993.1986.
PMID:8352802
Abstract
A chimeric cDNA was constructed, coding for the N-terminal region of BS-RNase (residues 1-49) and the C-terminal region of RNase A (residues 50-124). The resulting chimeric DNA was expressed in Escherichia coli and found to code for RNase chains that spontaneously assembled into a covalently dimeric ribonuclease.
摘要
构建了一个嵌合cDNA，其编码BS-RNase的N端区域（第1-49位氨基酸残基）和RNase A的C端区域（第50-124位氨基酸残基）。所得的嵌合DNA在大肠杆菌中表达，发现其编码的RNase链能自发组装成共价二聚体核糖核酸酶。
Zotero 插件