Troconiz J I, Lopez-Bustamante L G, Fos D
Departamento de Farmacia y Tecnología farmaceútica, Facultad de Farmacia, Universidad de Navarra, Pamplona, Spain.
Arzneimittelforschung. 1993 Jun;43(6):679-81.
A rapid and sensitive high-performance liquid chromatographic procedure is described for the simultaneous determination of piroxicam (CAS 36322-90-4) and tenoxicam (CAS 59804-37-4) in blood, plasma and buffer solutions used in the equilibrium dialysis studies. The method can be used to measure either piroxicam and tenoxicam in these three fluids using the other as internal standard. A Nucleosil C18 and a mobile phase consisting of an acetonitrile-distilled water-acetic acid (58 : 38 : 4) mixture were used. The flow rate was 1 ml/min and the effluent was monitored at 365 nm with 0.02 AUFS (absorbance units full scale). The sensitivities of this method were 0.2 microgram/ml levels of piroxicam and tenoxicam in the plasma, blood and buffer solutions samples.
本文描述了一种快速灵敏的高效液相色谱法，用于同时测定平衡透析研究中血液、血浆和缓冲溶液中的吡罗昔康（CAS 36322-90-4）和替诺昔康（CAS 59804-37-4）。该方法可使用另一种药物作为内标，来测定这三种液体中的吡罗昔康或替诺昔康。使用了Nucleosil C18柱和由乙腈-蒸馏水-乙酸（58:38:4）混合物组成的流动相。流速为1 ml/min，流出物在365 nm处用0.02 AUFS（满量程吸光度单位）进行监测。该方法对血浆、血液和缓冲溶液样品中吡罗昔康和替诺昔康的检测限为0.2微克/毫升。
