7q21-q32区域基因和DNA片段的精细定位及酵母人工染色体（YAC）重叠群图谱绘制

Refined localization and yeast artificial chromosome (YAC) contig--mapping of genes and DNA segments in the 7q21-q32 region.

作者信息

Scherer S W, Rommens J M, Soder S, Wong E, Plavsic N, Tompkins B J, Beattie A, Kim J, Tsui L C

机构信息

Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada.

出版信息

Hum Mol Genet. 1993 Jun;2(6):751-60. doi: 10.1093/hmg/2.6.751.

DOI:10.1093/hmg/2.6.751

PMID:8353494

Abstract

The chromosome localizations for 159 gene and DNA segments have been refined to one of five intervals in the 7q21-132 region through hybridization analysis with a panel of somatic cell hybrid lines. Seventy-two of these chromosome 7 markers are also mapped on common or overlapping yeast artificial chromosome (YAC) clones. In addition, the breakpoints of chromosome rearrangement contained in five of the somatic cell hybrid lines have been defined by flanking probes within YAC contigs. To provide a framework for further mapping of the 7q21-q32 region, we have established the physical order of a set of reference markers: cen-(COL1A2-D7S15-CYP3A4-PON)-D7S456-(brea kpoint contained in cell hybrid 1EF2/3/K017)-GUSB-D7S186-ASL-(PGY1-PGY3 -GNB2-EPO-ACHE)-D7S238-(proximal breakpoint in GM1059-Rag5)-D7S240-(CUTL1-PLANH1)-(breakp oints in 1CF2/5/K016 and 2068Rag22-2)-(PRKAR2B-D7S13)-LAMB1-(breakpoint in JSR-17S)-DLD-D7S16-MET-WNT2-CFTR-D7S8-tel.

摘要

通过与一组体细胞杂交系进行杂交分析，159个基因和DNA片段的染色体定位已被精确到7q21 - 132区域的五个区间之一。其中72个7号染色体标记也定位在常见或重叠的酵母人工染色体（YAC）克隆上。此外，五个体细胞杂交系中所含染色体重排的断点已通过YAC重叠群中的侧翼探针确定。为了为7q21 - q32区域的进一步定位提供框架，我们建立了一组参考标记的物理顺序：着丝粒 - （COL1A2 - D7S15 - CYP3A4 - PON） - D7S456 - （细胞杂交系1EF2/3/K017中包含的断点） - GUSB - D7S186 - ASL - （PGY1 - PGY3 - GNB2 - EPO - ACHE） - D7S238 - （GM1059 - Rag5中的近端断点） - D7S240 - （CUTL1 - PLANH1） - （1CF2/5/K016和2068Rag22 - 2中的断点） - （PRKAR2B - D7S13） - LAMB1 - （JSR - 17S中的断点） - DLD - D7S16 - MET - WNT2 - CFTR - D7S8 - 端粒。