Riethman H C, Spais C, Buckingham J, Grady D, Moyzis R K
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
Genomics. 1993 Jul;17(1):25-32. doi: 10.1006/geno.1993.1278.
DNA from a 240-kb human telomeric yeast artificial chromosome (HTY) clone was analyzed using physical mapping methods. Cosmid subclones of the YAC were fingerprinted using restriction enzyme digestion and human repeat sequence hybridization and then assembled into two contigs that together span 93% of the human insert. Data from restriction mapping and Bal31 exonuclease experiments indicate that, except for the truncation of distal genomic (T2AG3)n sequences, the molecular clone HTY 146 contains a contiguous, 230-kb telomere-terminal fragment from 7qter. Markers derived from this clone will allow telomeric closure of the physical and genetic linkage maps of human chromosome 7q.
使用物理作图方法分析了来自一个240kb人类端粒酵母人工染色体（HTY）克隆的DNA。利用限制性酶切和人类重复序列杂交对该YAC的黏粒亚克隆进行指纹分析，然后组装成两个重叠群，它们共同覆盖了人类插入片段的93%。限制性酶切图谱和Bal31核酸外切酶实验的数据表明，除了远端基因组（T2AG3）n序列的截短外，分子克隆HTY 146包含一个来自7qter的连续的230kb端粒末端片段。源自该克隆的标记将使人类染色体7q的物理图谱和遗传连锁图谱的端粒闭合成为可能。
