Pietrantonio P V, Gill S S
Department of Entomology, University of California, Riverside 92521.
Insect Biochem Mol Biol. 1993 Sep;23(6):675-80. doi: 10.1016/0965-1748(93)90041-p.
A 0.4 kb polymerase chain reaction (PCR) product obtained from cDNA made from the midgut and Malpighian tubules of fifth instar larvae of Heliothis virescens was used to screen a larval midgut and Malpighian tubules cDNA library. Four clones were obtained, one of 1.9 kb and others of 1.4 kb. The 1.9 kb clone encodes a 17.2 kDa protein which is highly homologous to other vacuolar ATPases proteolipids. Putative N-glycosylation and DCCD binding sites were observed at amino acid residues 83 and 139, respectively.
从绿铃夜蛾五龄幼虫中肠和马氏管制备的cDNA中获得的一段0.4 kb聚合酶链式反应（PCR）产物，用于筛选幼虫中肠和马氏管cDNA文库。获得了4个克隆，一个为1.9 kb，其他为1.4 kb。1.9 kb的克隆编码一种17.2 kDa的蛋白质，该蛋白质与其他液泡型ATP酶蛋白脂质高度同源。在氨基酸残基83和139处分别观察到推定的N-糖基化和DCCD结合位点。
