Scott J G, Lee S S
Department of Entomology, Cornell University, Ithaca, NY 14853-0999.
Insect Biochem Mol Biol. 1993 Sep;23(6):729-38. doi: 10.1016/0965-1748(93)90047-v.
Cytochrome P-450s, cytochrome b5, P-450 reductase, and four P-450 monooxygenase activities were abundant in proximal intestine (PI), Malpighian tubules (MT), and fat bodies (FB) in the insecticide resistant (LPR) strain of house fly. Insecticide resistance in LPR flies was associated with increases in P-450 monooxygenase activities in nearly all of these tissues relative to an insecticide susceptible strain. Cytochrome P-450lpr was present in all tissues and at higher levels in LPR than in susceptible flies suggesting that the role of P-450lpr in monooxygenase-mediated insecticide resistance is not due to its higher levels in a single tissue. However, it appears that fat bodies may have a major role in resistance due to their relatively large size and the abundance of P-450lpr in this tissue. Total cytochrome P-450s, cytochrome P-450lpr and P-450 monooxygenase activities were generally refractory to phenobarbital (PB) induction in LPR. This contrasts with a previous report in susceptible house flies showing that P-450 monooxygenases were induced by PB. It appears that total P-450s, cytochrome P-450lpr and monooxygenase activities were expressed at constitutive high levels in several tissues of LPR house flies.
细胞色素P-450、细胞色素b5、P-450还原酶以及四种P-450单加氧酶活性在抗杀虫剂（LPR）品系家蝇的近端肠道（PI）、马氏管（MT）和脂肪体（FB）中含量丰富。相对于杀虫剂敏感品系，LPR品系家蝇的抗药性与几乎所有这些组织中P-450单加氧酶活性的增加有关。细胞色素P-450lpr存在于所有组织中，且在LPR品系家蝇中的含量高于敏感品系家蝇，这表明P-450lpr在单加氧酶介导的抗药性中的作用并非因其在单一组织中的含量较高。然而，由于脂肪体相对较大且该组织中P-450lpr含量丰富，其似乎在抗药性中起主要作用。在LPR品系家蝇中，总细胞色素P-450、细胞色素P-450lpr和P-450单加氧酶活性通常对苯巴比妥（PB）诱导具有抗性。这与之前关于敏感家蝇的报道形成对比，该报道显示P-450单加氧酶可被PB诱导。似乎在LPR品系家蝇的多个组织中，总P-450、细胞色素P-450lpr和单加氧酶活性以组成型高水平表达。
