Miller R R, Yates J W, Geer B W
Department of Biology, Knox College, Galesburg, IL 61401.
Insect Biochem Mol Biol. 1993 Sep;23(6):749-55. doi: 10.1016/0965-1748(93)90049-x.
When administered in the diet to third instar Drosophila melanogaster larvae, short chain primary alcohols reduce phosphatidylcholine (PC) levels. The ethanol-induced reductions in larval PC may be in part due to an increase in the activity of PC-specific phospholipase D (PC-specific PLD, EC 3.1.4.4). PC-specific PLD not only hydrolyzes PC, but it also apparently catalyzes the formation of phosphatidylethanol. PC-specific PLD activity was also stimulated by 200 mM ethanol, methanol, isopropanol, n-butanol, and n-propanol. In vitro studies indicated that Drosophila PC-specific PLD activities were enhanced by submicromolar concentrations of Ca2+ and by GTP-gamma S. In vivo studies utilizing [14C]lyso-palmitoyl phosphatidylcholine indicated that dietary ethanol promoted the flux of label into triacylglycerol, 1,2 diacylglycerol, and fatty acid ethyl esters, while the label in PC decreased.
当在饮食中给予三龄黑腹果蝇幼虫时，短链伯醇会降低磷脂酰胆碱（PC）水平。乙醇诱导的幼虫PC水平降低可能部分归因于PC特异性磷脂酶D（PC特异性PLD，EC 3.1.4.4）活性的增加。PC特异性PLD不仅能水解PC，而且显然还催化磷脂酰乙醇的形成。200 mM乙醇、甲醇、异丙醇、正丁醇和正丙醇也能刺激PC特异性PLD活性。体外研究表明，亚微摩尔浓度的Ca2+和GTP-γS可增强果蝇PC特异性PLD活性。利用[14C]溶血棕榈酰磷脂酰胆碱进行的体内研究表明，饮食中的乙醇会促使标记物流入三酰甘油、1,2-二酰甘油和脂肪酸乙酯，而PC中的标记物则减少。
