Mamon L A, Kutskova Iu A
Genetika. 1993 Apr;29(4):604-12.
The synthesis of the heat-shock proteins (HSP) and the frequency of the mitotic chromosome damages (pulverizations, chromosome, chromatid stickiness, etc.) was studied in the cells of larva nervous ganglion in the process of recovery after heat shock (37 degrees C, 30 min). Analysis was performed in two strains of Drosophila melanogaster (Canton S-the wild type and the mutant l(1)ts403 with the defect in the HSP system). Correlation between the dynamics of HSP synthesis and the alteration of the chromosome pulverization and stickiness frequency was shown for both Drosophila strains under study. These results, in accord with the data on HSPs' binding to chromosomes following heat shock and their role as molecular chaperones, allowed to suggest that HSPs took part in chromosome condensation and in the recovery of heat shock induced damages leading to chromosome and chromatid stickiness.
在热休克（37摄氏度，30分钟）后的恢复过程中，研究了幼虫神经节细胞中热休克蛋白（HSP）的合成以及有丝分裂染色体损伤（粉碎、染色体、染色单体粘连等）的频率。对两种黑腹果蝇品系（野生型Canton S和HSP系统存在缺陷的突变体l(1)ts403）进行了分析。在所研究的两种果蝇品系中，均显示出HSP合成动力学与染色体粉碎和粘连频率变化之间的相关性。这些结果与热休克后HSP与染色体结合的数据及其作为分子伴侣的作用相一致，表明HSP参与了染色体凝聚以及热休克诱导的导致染色体和染色单体粘连的损伤的恢复过程。
