一种从化石骨骼中分离DNA以用于后续聚合酶链反应扩增的简单有效方法
[A simple and effective method for isolation of DNA from fossil bones for subsequent amplification using the polymerase chain reaction].
作者信息
Petrishchev V N, Kutueva A B, Rychkov Iu G
出版信息
Genetika. 1993 Apr;29(4):690-3.
PMID:8354476
Abstract
A method for DNA extraction from fossil bones based on DNA water elution from the bone powder is proposed. The method allows extraction of paleo-DNA from the great number of bone specimens simultaneously within one day and in quantities sufficient for DNA amplification by the polymerase chain reaction.
摘要
提出了一种基于从骨粉中进行DNA水洗脱来从化石骨骼中提取DNA的方法。该方法能够在一天内同时从大量骨骼标本中提取古DNA，且提取量足以用于通过聚合酶链反应进行DNA扩增。
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