Kannan V M, Appukuttan P S
Neurochemistry Division, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram.
Indian J Biochem Biophys. 1993 Apr;30(2):111-6.
Sugar-specific binding of the 14 kDa beta-galactoside-binding lectin from bovine brain grey matter to mixed endogenous gangliosides was demonstrated by affinity electrophoresis and hemagglutination inhibition. Gangliosides prepared by Folch extraction, base treatment and silica gel chromatography, when incorporated in native or desialated form in polyacrylamide gel above their critical micellar concentration, arrested the mobility of the lectin during electrophoresis at pH 8.2. This effect was sugar-specific since it was reversed if lactose, but not sucrose, was present in the gel. Also, retention of the brain lectin by ganglioside and its reversal by lactose were concentration-dependent. In presence of bovine serum albumin, at pH 7.4 native and desialylated gangliosides equally inhibited agglutination of trypsinized rabbit red cells by bovine brain lectin, but not that by the alpha-galactoside-specific antibody from human serum. Results suggested the possibility of endogenous gangliosides acting as cell surface receptors in mediation of brain lectin function.
通过亲和电泳和血凝抑制实验证明，牛脑灰质中14 kDa的β-半乳糖苷结合凝集素与混合内源性神经节苷脂存在糖特异性结合。用福尔克提取法、碱处理法和硅胶柱色谱法制备的神经节苷脂，当以天然或去唾液酸形式、高于其临界胶束浓度掺入聚丙烯酰胺凝胶中时，在pH 8.2的电泳过程中会阻止凝集素的迁移。这种效应具有糖特异性，因为如果凝胶中存在乳糖而非蔗糖，这种效应就会逆转。此外，神经节苷脂对脑凝集素的保留及其被乳糖逆转的过程也呈浓度依赖性。在牛血清白蛋白存在的情况下，在pH 7.4时，天然和去唾液酸的神经节苷脂均能同等程度地抑制牛脑凝集素对胰蛋白酶处理过的兔红细胞的凝集作用，但对人血清中α-半乳糖苷特异性抗体介导的凝集作用无抑制效果。结果提示内源性神经节苷脂有可能作为细胞表面受体介导脑凝集素的功能。
