Kambara T, McFarlane R G, Abell T J, McAnulty R W, Sykes A R
Animal and Veterinary Sciences Group, Lincoln University, Canterbury, New Zealand.
Int J Parasitol. 1993 Jul;23(4):471-6. doi: 10.1016/0020-7519(93)90035-w.
Development of immunity and resistance to Trichostrongylus colubriformis was measured in sheep, 8-26 or 33-51 weeks of age, that were offered two levels of dietary protein (11 and 20%). Resistance to challenge infection was ascertained by measuring worm burden, faecal egg count and eggs in utero in the nematode. Development of immunity was measured by an in vitro lymphocyte blastogenesis test to T and B cell mitogens and to somatic antigen from infective (L3) larvae. Young lambs offered the low protein diet showed a significantly lower resistance to parasites than older animals. The young animals on the high protein diet developed better resistance. Age and dietary protein influenced in vitro T lymphocyte responses to parasite antigen and mitogens, which were particularly well developed in vaccinated lambs on the high protein diet. Lymphocyte responsiveness to L3 larval antigen was negatively correlated with parasite status in vaccinated older lambs, and may be important in the development of acquired resistance to T. colubriformis.
对8至26周龄或33至51周龄的绵羊进行了对蛇形毛圆线虫免疫力和抵抗力的研究，这些绵羊被给予两种水平的膳食蛋白质（11%和20%）。通过测量线虫的虫体负荷、粪便虫卵计数和子宫内虫卵来确定对攻击感染的抵抗力。通过体外淋巴细胞对T和B细胞有丝分裂原以及感染性（L3）幼虫体细胞抗原的增殖试验来测量免疫力的发展。食用低蛋白饮食的幼龄羔羊对寄生虫的抵抗力明显低于老龄动物。食用高蛋白饮食的幼龄动物产生了更好的抵抗力。年龄和膳食蛋白质影响体外T淋巴细胞对寄生虫抗原和有丝分裂原的反应，在食用高蛋白饮食的接种疫苗的羔羊中这种反应尤为明显。在接种疫苗的老龄羔羊中，淋巴细胞对L3幼虫抗原的反应性与寄生虫状态呈负相关，这可能对获得性抗蛇形毛圆线虫抵抗力的发展很重要。
