The optimisation of a novel iodide microassay and its application in an immunoassay for human antibody levels in serum.
作者信息
O'Kennedy R, Keating P
机构信息
School of Biological Sciences, Dublin City University, Ireland.
出版信息
J Immunol Methods. 1993 Aug 9;163(2):225-31. doi: 10.1016/0022-1759(93)90126-r.
PMID:8354891
Abstract
Radioactive iodine has been used as an antibody label in many immunoassays. The feasibility of using non-radioactive iodine as a label was investigated. A microassay for iodide based on the Sandell-Kolthoff reaction was optimised. It was used to detect iodine-labelled antibody and successfully applied to the detection of human IgG in serum samples. The performance of this assay system compared with enzyme-linked immunosorbent assay using HRP was evaluated.
摘要
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