Patel D, Rubbi C P, Rickwood D
Department of Biology, University of Essex, Colchester, England, UK.
J Immunol Methods. 1993 Aug 9;163(2):241-51. doi: 10.1016/0022-1759(93)90128-t.
Experiments have been carried out to demonstrate that, using antibody coated-Dynabeads as a model system for density labelling MOLT-4 T cells, the overall density of cells can be increased such that the cells that bind particles can be separated on isopycnic isotonic density gradients from cells that bind fewer particles. The increase in density is dependent on the cell volume and the number of particles bound. After centrifugation, cells with bound particles were found at positions in the gradient that reflected their increased density. Observed density ranges for cells with particular numbers of particles bound coincided closely with calculated expected density ranges. These results indicate the potential for separation of different subpopulations of cells on the basis of the immunological identity of the surface of cells using density perturbation methods involving antibody coated-density particles.
已经进行了实验以证明，使用包被抗体的磁珠作为对MOLT-4 T细胞进行密度标记的模型系统，可以增加细胞的整体密度，从而使结合颗粒的细胞能够在等密度等渗密度梯度上与结合较少颗粒的细胞分离。密度的增加取决于细胞体积和结合的颗粒数量。离心后，发现结合颗粒的细胞位于梯度中的相应位置，这反映了它们增加的密度。观察到的结合特定数量颗粒的细胞的密度范围与计算出的预期密度范围非常吻合。这些结果表明，使用涉及包被抗体的密度颗粒的密度扰动方法，有可能基于细胞表面的免疫特性分离不同的细胞亚群。
