Patel D, Rubbi C P, Rickwood D
Department of Biology, University of Essex, Wivenhoe Park, Colchester, UK.
Clin Chim Acta. 1995 Sep 15;240(2):187-93. doi: 10.1016/0009-8981(95)06141-9.
The fractionation of sub-populations of peripheral blood mononuclear cells has become an essential routine procedure and some of the main fractionation methods used today are immunomagnetic separations. We describe a less expensive method for the separation of subpopulations of mononuclear cells using density perturbation, which uses the binding of antibody-coated dense polystyrene beads to increase the density of specific sub-populations of cells. By incubating a total mononuclear fraction from human peripheral blood together with antibody-coated beads, in a commercially-available lymphocyte separation medium (Nycoprep 1.077), a depletion of 94.9 +/- 1.68% of the T cells could be obtained by this procedure; a depletion of 69.7 +/- 1.78% of the B cells was also achieved. These results indicate the potential for the separation of different sub-populations of peripheral blood mononuclear cells on the basis of the immunological identity of the surface of cells using density perturbation methods involving antibody-coated dense polystyrene beads.
外周血单个核细胞亚群的分离已成为一项基本的常规操作，当今使用的一些主要分离方法是免疫磁珠分离法。我们描述了一种使用密度扰动分离单个核细胞亚群的成本较低的方法，该方法利用包被抗体的致密聚苯乙烯珠的结合来增加特定细胞亚群的密度。通过将人外周血的总单个核细胞组分与包被抗体的珠子一起在市售的淋巴细胞分离培养基（Nycoprep 1.077）中孵育，通过该程序可使T细胞减少94.9±1.68%；B细胞也减少了69.7±1.78%。这些结果表明，利用涉及包被抗体的致密聚苯乙烯珠的密度扰动方法，基于细胞表面的免疫学特性分离外周血单个核细胞不同亚群具有潜力。
