Hesselton R M, Koup R A, Cromwell M A, Graham B S, Johns M, Sullivan J L
University of Massachusetts Medical School, Dept. of Pediatrics, Worcester 01605.
J Infect Dis. 1993 Sep;168(3):630-40. doi: 10.1093/infdis/168.3.630.
Immune reconstitutions (hu-PBL-SCID mice) resulting from adoptive transfer of human peripheral blood mononuclear cells into 1800 C.B-17 scid-/scid-mice were characterized. Over 90% of reconstitutions were successful as evidenced by human immunoglobulin production. Variability was noted with donor, cell number, and cell type. Human cells (T lymphocytes, few B cells) could be recovered by 5 days after engraftment. High levels of soluble CD8 and interleukin-2 receptors were detected in sera of hu-PBL-SCID mice. Cells recovered from 17 mice proliferated in response to antigens to which the donor had been primed; responses to nonboosted antigen also increased in some animals. After reconstitution, lymphocytes were found in the spleen and lymph nodes without full restoration of normal architecture. The hu-PBL-SCID mouse shows promise as a model system for a variety of immunologic studies. The inherent variation in the system must be minimized for appropriate use of the model.
对将人外周血单个核细胞过继转移至1800只C.B-17 scid-/scid小鼠所产生的免疫重建（人外周血淋巴细胞-严重联合免疫缺陷小鼠）进行了特征分析。超过90%的重建是成功的，这可通过人免疫球蛋白的产生得以证明。注意到供体、细胞数量和细胞类型存在变异性。移植后5天即可回收人细胞（T淋巴细胞，少量B细胞）。在人外周血淋巴细胞-严重联合免疫缺陷小鼠的血清中检测到高水平的可溶性CD8和白细胞介素-2受体。从17只小鼠回收的细胞对供体已致敏的抗原产生增殖反应；在一些动物中，对未加强免疫的抗原的反应也有所增加。重建后，在脾脏和淋巴结中发现了淋巴细胞，但正常结构未完全恢复。人外周血淋巴细胞-严重联合免疫缺陷小鼠有望成为用于各种免疫学研究的模型系统。为了适当地使用该模型，必须将该系统中固有的变异性降至最低。
