Olive C, Cheung C, Falk M C
Renal Research Laboratory, Renal Dialysis Unit, Princess Alexandra Hospital, Woolloongabba, Brisbane, Queensland, Australia.
Immunol Cell Biol. 1998 Dec;76(6):520-5. doi: 10.1046/j.1440-1711.1998.00786.x.
The reconstitution of severe combined immunodeficiency (SCID) mice with human PBL (Hu-PBL-SCID) was assessed using fresh unstimulated PBL and anti-CD3-stimulated PBL. Mice were reconstituted with PBL by intraperitoneal injection of 1-2.5 x 107 PBL in PBS; controls received PBS. Successful engraftment of human PBL in SCID mice was determined by measurement of human IgG in mouse sera, polymerase chain reaction (PCR) detection of human-specific HLA-DRbeta DNA in SCID periphery, and immunohistochemical staining of mouse tissues (spleen, lymph nodes, thymus, liver and lung) with antibodies specific for human CD45 and CD3. Human IgG was detected 1 week after reconstitution in sera of all animals that received at least 1 x 107 PBL and continued to increase for 8 weeks. Human-specific HLA-DRbeta DNA was detected in the majority of mice 3 weeks after reconstitution but not in controls. Moreover, immunohistochemical analysis of Hu-PBL-SCID mouse tissues revealed the presence of human CD45+ cells in all tissues examined. CD3+ T cell engraftment was observed in lymphoid tissues irrespective of whether PBL had been activated prior to transfer or not.
使用新鲜未刺激的外周血淋巴细胞（PBL）和抗CD3刺激的PBL评估严重联合免疫缺陷（SCID）小鼠与人PBL重建的人源化SCID小鼠（Hu-PBL-SCID）。通过在PBS中腹腔注射1-2.5×10⁷个PBL用PBL重建小鼠；对照组接受PBS。通过测量小鼠血清中的人IgG、聚合酶链反应（PCR）检测SCID外周血中人类特异性HLA-DRβ DNA以及用针对人CD45和CD3的特异性抗体对小鼠组织（脾脏、淋巴结、胸腺、肝脏和肺）进行免疫组织化学染色来确定人PBL在SCID小鼠中的成功植入。在所有接受至少1×10⁷个PBL的动物血清中，重建后1周检测到人类IgG，并持续增加8周。重建后3周，大多数小鼠中检测到人类特异性HLA-DRβ DNA，但对照组未检测到。此外，Hu-PBL-SCID小鼠组织的免疫组织化学分析显示，在所检查的所有组织中均存在人CD45⁺细胞。无论PBL在转移前是否被激活，在淋巴组织中均观察到CD3⁺ T细胞植入。
