铜绿假单胞菌环己二烯基脱水酶的初步晶体学研究。

Preliminary crystallographic study of cyclohexadienyl dehydratase from Pseudomonas aeruginosa.

作者信息

Obmolova G, Teplyakov A, Xia T, Jensen R

机构信息

EMBL, Hamburg, Germany.

出版信息

J Mol Biol. 1993 Aug 5;232(3):992-4. doi: 10.1006/jmbi.1993.1446.

DOI:10.1006/jmbi.1993.1446

PMID:8355283

Abstract

Single crystals of cyclohexadienyl dehydratase from Pseudomonas aeruginosa have been obtained by vapour diffusion from ammonium sulphate solution (pH 6.0) at 4 degrees C. The crystals belong to the tetragonal space group P4(3)2(1)2 or P4(1)2(1)2 with a = b = 105.5 A and c = 165.0 A. The asymmetric unit contains at least one dimeric protein molecule with M(r) = 72 kDa. The crystals diffract to 3 A resolution and are suitable for an X-ray analysis.

摘要

通过在4℃下从硫酸铵溶液（pH 6.0）中进行气相扩散，已获得了铜绿假单胞菌环己二烯基脱水酶的单晶。这些晶体属于四方晶系空间群P4(3)2(1)2或P4(1)2(1)2，a = b = 105.5 Å，c = 165.0 Å。不对称单元至少包含一个分子量为72 kDa的二聚体蛋白质分子。这些晶体的衍射分辨率可达3 Å，适合进行X射线分析。