铜绿假单胞菌蛋白酶的结晶及初步X射线研究
Crystallization and preliminary X-ray studies of a protease from Pseudomonas aeruginosa.
作者信息
Harada S, Nagara A, Kurisu G, Kai Y
机构信息
Department of Applied Chemistry, Faculty of Engineering, Osaka University, Japan.
出版信息
J Mol Biol. 1993 Apr 20;230(4):1315-6. doi: 10.1006/jmbi.1993.1247.
PMID:8487310
Abstract
A protease produced by Pseudomonas aeruginosa has been crystallized by the vapor-diffusion method using polyethylene glycol 4000 as a precipitant. The crystals belong to the hexagonal space group P6(1) (P6(5) with unit cell dimensions; a = b = 106.9 A, c = 96.9 A. There are two molecules per asymmetric unit. The crystals diffract X-rays to at least 3.0 A and are suitable for X-ray crystallographic studies.
摘要
铜绿假单胞菌产生的一种蛋白酶已经通过气相扩散法，以聚乙二醇4000作为沉淀剂进行了结晶。这些晶体属于六方空间群P6(1)（P6(5)），晶胞参数为：a = b = 106.9 Å，c = 96.9 Å。每个不对称单元中有两个分子。这些晶体的X射线衍射能力至少可达3.0 Å，适合进行X射线晶体学研究。
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