Ito J, Kamiya Y, Kawaguchi M
The Third Department of Internal Medicine, Nagoya City University Medical School, Japan.
Life Sci. 1993;53(9):717-24. doi: 10.1016/0024-3205(93)90248-2.
We evaluated the content of EGF in platelet lysates obtained from 49 patients with non-insulin-dependent diabetes mellitus (NIDDM)(18 males, 31 females, age 58 +/- 13 years) and from 23 clinically healthy control subjects (11 males, 12 females, age 53 +/- 18 years). Platelets were collected from platelet-rich plasma and lysed. EGF was determined by radioimmunoassay. The immunoreactive EGF content in the platelet lysates in diabetic patients significantly exceeded that of control subjects (44.9 +/- 18.5 pg/mm3platelet vs. 34.2 +/- 7.8 pg/mm3platelet, mean +/- SD p < 0.008). In performing multiple regression analysis with ten clinical parameters, urinary albumin excretion (F = 16.1, r = 0.551, p < 0.001), duration of diabetes (F = 13.0, r = 0.511, p < 0.001) and the presence of diabetic proliferative retinopathy (F = 8.8, p < 0.01) were significantly associated with irEGF content in platelet lysates. These observations suggest that the amount of EGF in platelets may increase with the progression of diabetic complications. The mechanism for the increase of EGF in platelets remains to be clarified.
我们评估了从49例非胰岛素依赖型糖尿病（NIDDM）患者（18例男性，31例女性，年龄58±13岁）和23例临床健康对照者（11例男性，12例女性，年龄53±18岁）获取的血小板裂解物中表皮生长因子（EGF）的含量。从富含血小板的血浆中采集血小板并进行裂解。通过放射免疫测定法测定EGF。糖尿病患者血小板裂解物中免疫反应性EGF含量显著超过对照者（44.9±18.5 pg/mm³血小板 vs. 34.2±7.8 pg/mm³血小板，均值±标准差，p<0.008）。在对10项临床参数进行多元回归分析时，尿白蛋白排泄量（F = 16.1，r = 0.551，p<0.001）、糖尿病病程（F = 13.0，r = 0.511，p<0.001）以及糖尿病增殖性视网膜病变的存在（F = 8.8，p<0.01）与血小板裂解物中免疫反应性EGF含量显著相关。这些观察结果表明，血小板中EGF的量可能随着糖尿病并发症的进展而增加。血小板中EGF增加的机制仍有待阐明。
