Lev-Ran A, Hwang D L, Snyder D S
Department of Diabetes, Endocrinology, and Metabolism, City of Hope National Medical Center, Duarte, California 91010.
Am J Physiol. 1990 Sep;259(3 Pt 2):R545-8. doi: 10.1152/ajpregu.1990.259.3.R545.
Epidermal growth factor (EGF) was determined by radioimmunoassay in serum, plasma, and urine of 23 patients undergoing ablative therapy followed by bone marrow transplantation. The difference between the serum and plasma values reflected the amount of EGF released from the platelets at the time of blood coagulation. Platelet-derived EGF strongly correlated with platelet count (r + 0.850, P less than 0.0001), and the intercept of the regression line was very close to zero; one platelet contained approximately 2.5 x 10(-18) g EGF. Correspondingly, when the platelet count dropped after bone marrow ablation from 222 +/- 97 to 33 +/- 13 x 10(9)/l, the serum EGF decreased from 603 +/- 222 to 65 +/- 41 pg/ml (P less than 0.0001). Plasma EGF content did not correlate with the platelet count and did not change significantly after bone marrow ablation (before and after the ablation, correspondingly, 290 +/- 80 and 332 +/- 99 pg/ml, P = 0.194). High-performance liquid chromatographic fractionation of serum and plasma showed different molecular mass distribution of EGF-immunoreactive fractions. The main molecular mass components of the plasma EGF did not change after bone marrow ablation. Urine excretion remained unchanged (320 +/- 133 and 314 +/- 173 pmol EGF/mmol creatinine). We conclude that whereas platelets are the source of serum EGF, the origin of plasma EGF is different and the search of its origin is warranted.
采用放射免疫分析法对23例接受骨髓移植的清髓性治疗患者的血清、血浆和尿液中的表皮生长因子（EGF）进行了测定。血清和血浆值之间的差异反映了凝血时从血小板释放的EGF量。血小板源性EGF与血小板计数密切相关（r = +0.850，P<0.0001），回归线的截距非常接近零；一个血小板约含2.5×10⁻¹⁸g EGF。相应地，骨髓消融后血小板计数从222±97降至33±13×10⁹/L时，血清EGF从603±222降至65±41 pg/ml（P<0.0001）。血浆EGF含量与血小板计数无关，骨髓消融后无显著变化（消融前后分别为290±80和332±99 pg/ml，P = 0.194）。血清和血浆的高效液相色谱分离显示EGF免疫反应性组分的分子量分布不同。骨髓消融后血浆EGF的主要分子量成分未改变。尿排泄量保持不变（320±133和314±173 pmol EGF/mmol肌酐）。我们得出结论，血小板是血清EGF的来源，而血浆EGF的来源不同，有必要对其来源进行研究。
