el-Farrash M A, Masuda T, Kuroda M J, Harada S
Department of Biodefence and Medical Virology, Kumamoto University School of Medicine, Japan.
Microbiol Immunol. 1993;37(5):349-57. doi: 10.1111/j.1348-0421.1993.tb03221.x.
The effect of host cell factors on infectivity of human immunodeficiency virus type 1 (HIV-1) was studied by infecting a monoblastoid cell line (U937) or a T-cell line (MOLT-4) with a highly infective single clone of HIV-1 and comparing the infectivity of the produced viruses to different cell lines. Chronically infected U937 cells consistently produced viruses with minimal infectivity. This phenotypic change was host-dependent as the back-passage of the U937-produced low infective viruses into MOLT-4 cells resulted in regaining their original high infectivity. Southern and Northern blot analyses of the HIV-1 grown in U937 cells did not reveal any genomic difference between it and the virus grown it MOLT-4 cells. The radioimmunoprecipitation analysis of viral proteins showed that the HIV-1-infected U937 cells had a different pattern of envelope glycoproteins and core proteins, which well correlated with the low infectivity of the produced viruses. This experimental system using MOLT-4 and U937 cell lines would be useful to further explore host cell factor(s) which play an important role in the regulation of HIV-1 infectivity.
通过用高感染性的HIV-1单克隆感染单核细胞系（U937）或T细胞系（MOLT-4），并比较产生的病毒对不同细胞系的感染性，研究了宿主细胞因子对1型人类免疫缺陷病毒（HIV-1）感染性的影响。长期感染的U937细胞持续产生感染性极低的病毒。这种表型变化是宿主依赖性的，因为将U937产生的低感染性病毒回传至MOLT-4细胞会使其恢复原来的高感染性。对在U937细胞中生长的HIV-1进行Southern和Northern印迹分析，未发现其与在MOLT-4细胞中生长的病毒之间存在任何基因组差异。病毒蛋白的放射免疫沉淀分析表明，感染HIV-1的U937细胞具有不同的包膜糖蛋白和核心蛋白模式，这与产生的病毒的低感染性密切相关。使用MOLT-4和U937细胞系的这个实验系统将有助于进一步探索在HIV-1感染性调节中起重要作用的宿主细胞因子。
