Bianciardi G, Tanganelli P, Weber G
Istituto di Anatomia ed Istologia Patologica, Università di Siena, Italy.
Semin Thromb Hemost. 1993;19(2):108-14. doi: 10.1055/s-2007-994013.
The new computerized approaches for image analysis available for a few years permit, in an excellent and detailed way, monitoring cellular structural modifications during activation processes. Here, we presented morphometric data obtained on blood cells (platelets, monocytes, and PMN cells) after in vitro activation and under in vivo conditions related to atherosclerosis studied by means of electron microscopy. Area and perimeter form factors, nucleus to cytoplasma area ratio, and the surface density of platelet plasma membrane openings of SCCS presented significant differences compared with the respective significant differences compared with the respective controls. Ultrastructural markers of blood cell activation were revealed by the computerized morphometry applied on ultrastructural images.
近年来出现的新型计算机化图像分析方法，能够以出色且详细的方式监测细胞在激活过程中的结构变化。在此，我们展示了通过电子显微镜研究的，体外激活后以及与动脉粥样硬化相关的体内条件下血细胞（血小板、单核细胞和多形核白细胞）的形态测量数据。与各自的对照组相比，SCCS的面积和周长形态因子、核质面积比以及血小板质膜开口的表面密度均存在显著差异。通过对超微结构图像应用计算机化形态测量法，揭示了血细胞激活的超微结构标记。
