Kageoka T, Kanekuni Y, Ueta T, Ogura K, Sugihara S, Nishiya K, Yamasaki F
Department of Clinical Laboratory Medicine, Kochi Medical School, Nankoku.
Rinsho Byori. 1996 Aug;44(8):757-63.
Platelet activation in addition to blood coagulation abnormality is regarded as a primary factor of thrombosis by atherosclerotic obstruction. Therefore, the platelet activation on atherosclerosis is considered to correlate with shear stress generated between blood cells and endothelial cells in blood flow. P-selectin on the surface of the platelet membrane is measured by flowcytometry as a marker of platelet activation. Herein, we examined the phenomena of platelet activation and adherent platelets with leukocytes (ad-P.L) on stored platelet concentration (PC) and chronic rheumatoid arthritis (RA), moreover, of the adherent platelets or polymorphonuclear cells (PMN) with endothelial cells (EC) in shear stress by using an apparatus we devised. The rate of platelet activation and ad-P.L increased in PC with storage, and the sensitivity of platelets to thrombin decreased. The rate of platelet activation and ad-P.L increased in RA in vivo. Many adherent platelets with EC were found at a low shear rate on normal EC but at a high shear rate on denatured EC without any specific adherent property. Adherent PMN with EC had several hundred times more denatured EC than normal EC and the relationship with shear rate disappeared on denatured EC. The platelet activation and relationship between platelets or leukocytes and EC as to the cause of thrombosis are important subjects for future studies.
除凝血异常外，血小板活化被认为是动脉粥样硬化阻塞导致血栓形成的主要因素。因此，血小板在动脉粥样硬化中的活化被认为与血流中血细胞和内皮细胞之间产生的剪切应力相关。通过流式细胞术测量血小板膜表面的P-选择素作为血小板活化的标志物。在此，我们使用自行设计的仪器，研究了储存血小板浓缩物（PC）和慢性类风湿性关节炎（RA）中血小板活化现象以及血小板与白细胞的黏附（ad-P.L），此外还研究了在剪切应力下血小板或多形核细胞（PMN）与内皮细胞（EC）的黏附情况。随着储存时间的延长，PC中血小板活化率和ad-P.L增加，血小板对凝血酶的敏感性降低。体内RA中血小板活化率和ad-P.L增加。在正常内皮细胞上，在低剪切速率下发现许多血小板与内皮细胞黏附，但在变性内皮细胞上，在高剪切速率下发现黏附，且无任何特异性黏附特性。与正常内皮细胞相比，PMN与变性内皮细胞的黏附数量多出数百倍，并且在变性内皮细胞上与剪切速率的关系消失。血小板活化以及血小板或白细胞与内皮细胞之间关于血栓形成原因的关系是未来研究的重要课题。
