Mitchell B S, Song J J, Johnson E E, Chen E, Dayton J S
University of North Carolina, Chapel Hill 27599.
Adv Enzyme Regul. 1993;33:61-8. doi: 10.1016/0065-2571(93)90009-3.
The human deoxycytidine kinase gene is a single copy gene and is comprised of seven exons that are spread over more than 34 kb of the genome. The 5'-flanking region is highly G/C rich and does not contain CAAT or TATA boxes. This region, when cloned into a recorder gene construct containing the chloramphenicol acetyltransferase gene, is capable of mediating CAT activity in human lymphoid cell lines and appears to have greater activity in human T, as compared to B, lymphoblast cell lines. The expression of the gene at the mRNA level does not appear to be cell-cycle regulated in that the levels of mRNA in human peripheral blood T lymphocytes remain constant as the cells progress from a resting to a proliferating state. Since this enzyme catalyzes the conversion of a number of chemotherapeutic agents to their corresponding monophosphate form and is thus essential for their activation, it will be important to define further the genetic elements which regulate the expression of this gene.
人类脱氧胞苷激酶基因是一个单拷贝基因，由七个外显子组成，分布在超过34 kb的基因组中。其5'侧翼区域富含G/C，不含CAAT或TATA框。该区域克隆到含有氯霉素乙酰转移酶基因的报告基因构建体中时，能够在人淋巴样细胞系中介导氯霉素乙酰转移酶（CAT）活性，并且与B淋巴母细胞系相比，在人T淋巴母细胞系中似乎具有更高的活性。该基因在mRNA水平的表达似乎不受细胞周期调控，因为当人外周血T淋巴细胞从静止状态进入增殖状态时，其mRNA水平保持恒定。由于这种酶催化多种化疗药物转化为其相应的单磷酸形式，因此对于它们的激活至关重要，进一步确定调节该基因表达的遗传元件将很重要。
