Wood J I, Klomparens K L
Center for Electron Optics, Michigan State University, East Lansing 48824.
Microsc Res Tech. 1993 Jul 1;25(4):267-75. doi: 10.1002/jemt.1070250402.
Agarose, agar, and gelatin were initially compared as encapsulation media for 3 structurally diverse particulate specimens: bacteria, yeast, and mitochondria. Agarose proved superior to both gelatin and agar for ease of handling and overall image quality (minimum background). All sample types exhibited high quality fixation and structural detail with no heat damage from the agarose medium. Based on this finding, we further characterized agarose encapsulation as affected by post-fixation, en bloc staining and resin type. Osmium tetroxide post-fixation, followed by en bloc uranyl acetate staining, could be performed without an increase in the electron density of the encapsulation medium. Agarose proved successful as an encapsulation medium regardless of the resin type or preparation protocol, thus providing flexibility in experimental design and excellent results over a range of variables.
最初，对琼脂糖、琼脂和明胶作为3种结构不同的颗粒标本（细菌、酵母和线粒体）的包封介质进行了比较。在易于操作和整体图像质量（最小背景）方面，琼脂糖被证明优于明胶和琼脂。所有样本类型均表现出高质量的固定和结构细节，且琼脂糖介质未造成热损伤。基于这一发现，我们进一步研究了后固定、整体染色和树脂类型对琼脂糖包封的影响。四氧化锇后固定，接着进行醋酸双氧铀整体染色，可以在不增加包封介质电子密度的情况下进行。无论树脂类型或制备方案如何，琼脂糖都被证明是一种成功的包封介质，从而在实验设计中提供了灵活性，并在一系列变量中取得了优异的结果。
