Schopf R E, Ockenfels H M, Schultewolter T, Morsches B
Department of Dermatology, Johannes Gutenberg University, Mainz, FRG.
Dermatology. 1993;187(2):100-3. doi: 10.1159/000247215.
Chloroquine is known to exacerbate psoriasis. Since immunological stimuli are considered to be important for the pathogenesis of psoriasis, we compared the effects of chloroquine on cell-mediated immunity in 15 healthy control individuals and 15 patients with psoriasis. We employed the spontaneous and phytohemagglutin (PHA)-induced uptake of 3H-thymidine to measure lymphocyte proliferation. Chloroquine was added to the cultures at concentrations ranging from 0.022 to 220 microM. We found that both spontaneous and PHA-driven lymphocyte proliferations were significantly lower in patients with psoriasis (p < 0.002). The spontaneous blastogenesis in both controls and patients remained stable under chloroquine. In PHA-driven cultures in controls, 0.022-2.2 microM chloroquine had no effect, higher concentrations of the drug suppressed proliferation. In patients, 22 microM chloroquine surmounted the suppression of the PHA-induced proliferative response found in controls; moreover, 2.2-0.022 microM chloroquine increased lymphocyte proliferation by > 300% (p < 0.002). Our data indicate that in psoriasis the lower lymphocyte transformation is abnormally stimulated by the addition of pharmacological doses of chloroquine.
已知氯喹会加重银屑病。由于免疫刺激被认为对银屑病的发病机制很重要，我们比较了氯喹对15名健康对照个体和15名银屑病患者细胞介导免疫的影响。我们采用自发和植物血凝素（PHA）诱导的3H-胸腺嘧啶摄取来测量淋巴细胞增殖。将氯喹以0.022至220微摩尔的浓度添加到培养物中。我们发现，银屑病患者的自发和PHA驱动的淋巴细胞增殖均显著降低（p < 0.002）。在氯喹作用下，对照者和患者的自发母细胞形成均保持稳定。在对照者的PHA驱动培养物中，0.022 - 2.2微摩尔的氯喹无作用，更高浓度的该药物抑制增殖。在患者中，22微摩尔的氯喹克服了对照者中PHA诱导的增殖反应抑制；此外，2.2 - 0.022微摩尔的氯喹使淋巴细胞增殖增加超过300%（p < 0.002）。我们的数据表明，在银屑病中，药理剂量的氯喹添加会异常刺激较低的淋巴细胞转化。
