Kumar S, Pritchard D I
Department of Life Science, University of Nottingham, University Park.
Biochem Mol Biol Int. 1993 May;30(1):13-9.
Two protease populations have been identified in the somatic products of adult Necator americanus. One population (158, 138, 34 and 31 kDa) loses, whereas the other population (107, 74, 51 and 20 kDa) retains, its proteolytic activity after elution from substrate gels. The present study warns that hookworm proteases may oligomerise and/or lose their activity under the conditions necessary for their purification. We suggest that following assessment of protease activity on substrate gels, the protease of interest should be eluted from plain gels; their isolation from substrate gels may lead to a reduced or no yield.
在美洲板口线虫成虫的体细胞产物中已鉴定出两种蛋白酶群体。一种群体（158、138、34和31 kDa）在从底物凝胶上洗脱后失去其蛋白水解活性，而另一种群体（107、74、51和20 kDa）则保留该活性。本研究警示，钩虫蛋白酶在其纯化所需的条件下可能会发生寡聚化和/或失去活性。我们建议，在底物凝胶上评估蛋白酶活性之后，应从普通凝胶上洗脱感兴趣的蛋白酶；从底物凝胶上分离它们可能会导致产量降低或无产量。
