Xiao Shuhua, Zhan Bin, Xue Jian, Goud Gaddam Narsa, Loukas Alex, Liu Yueyuan, Williamson Angela, Liu Sen, Deumic Vehid, Hotez Peter
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.
Exp Parasitol. 2008 Jan;118(1):32-40. doi: 10.1016/j.exppara.2007.05.010. Epub 2007 Jun 6.
We have previously reported the successful adaptation of human hookworm Necator americanus in the golden hamster, Mesocricetus auratus. This animal model was used to test a battery of hookworm (N. americanus and Ancylostoma caninum) recombinant antigens as potential vaccine antigens. Hamsters immunized a leading vaccine candidate N. americanus-Ancylostoma secreted protein 2 (Na-ASP-2) and challenged with N. americanus infective larvae (L3), resulted in 30-46.2% worm reduction over the course of three vaccine trials, relative to adjuvant controls. In addition, significant reduction of worm burdens was also observed in the hamsters immunized with adult hookworm antigens A. caninum aspartic protease 1 (Ac-APR-1); A. caninum-glutathione-S transferase 1 (Ac-GST-1) and Necator cysteine proteases 2 (Na-CP-2) (44.4%, 50.6%, and 29.3%, respectively). Our data on the worm burden reductions afforded by these hookworm antigens approximate the level of protection reported previously from dogs challenged with A. caninum L3, and provide additional evidence to support these hookworm antigens as vaccine candidates for human hookworm infection. The hamster model of N. americanus provides useful information for the selection of antigens to be tested in downstream vaccine development.
我们之前曾报道过成功使美洲板口线虫（Necator americanus）在金黄仓鼠（Mesocricetus auratus）体内适应。该动物模型用于测试一系列钩虫（美洲板口线虫和犬钩口线虫）重组抗原作为潜在疫苗抗原。用主要的候选疫苗美洲板口线虫 - 犬钩口线虫分泌蛋白2（Na - ASP - 2）免疫仓鼠，并使其感染美洲板口线虫感染性幼虫（L3），在三项疫苗试验过程中，相对于佐剂对照组，蠕虫减少率达30% - 46.2%。此外，在用犬钩口线虫天冬氨酸蛋白酶1（Ac - APR - 1）、犬钩口线虫 - 谷胱甘肽 - S转移酶1（Ac - GST - 1）和美洲板口线虫半胱氨酸蛋白酶2（Na - CP - 2）等成虫钩虫抗原免疫的仓鼠中，也观察到蠕虫负荷显著降低（分别为44.4%、50.6%和29.3%）。我们关于这些钩虫抗原所带来的蠕虫负荷降低的数据接近先前报道的用犬钩口线虫L3攻击的犬的保护水平，并为支持这些钩虫抗原作为人类钩虫感染的候选疫苗提供了额外证据。美洲板口线虫的仓鼠模型为下游疫苗开发中待测试抗原的选择提供了有用信息。
