Nesaretnam K, Devasagayam T P, Singh B B, Basiron Y
Palm Oil Research Institute of Malaysia, Bangi, Selangor Darul Ehsan.
Biochem Mol Biol Int. 1993 May;30(1):159-67.
The effect of palm oil, a widely used vegetable oil, rich in tocotrienols, on peroxidation potential of rat liver was examined. Long-term feeding of rats with palm oil as one of the dietary components significantly reduced the peroxidation potential of hepatic mitochondria and microsomes. As compared to hepatic mitochondria isolated from rats fed control or corn oil-rich diet, those from palm oil-fed group showed significantly less susceptibility to peroxidation induced by ascorbate and NADPH. However, in microsomes, only NADPH-induced lipid peroxidation was significantly reduced in rats fed palm oil rich-diet. Though the accumulation of thiobarbituric acid reactive substances during ascorbate-induced lipid peroxidation in mitochondria from rats fed corn oil-rich diet supplemented with tocotrienol-rich fraction (TRF) of palm oil was similar to that of control rats, the initial rate of peroxidation was much slower than those from control or corn oil fed diets. Our in vitro studies as well as analyses of co-factors related to peroxidation potential indicated that the observed decrease in palm oil-fed rats may be due to increased amount of antioxidants in terms of tocotrienol as well as decrease in the availability of substrates for peroxidation.
研究了广泛使用的富含生育三烯酚的植物油——棕榈油对大鼠肝脏过氧化潜能的影响。长期将棕榈油作为饮食成分之一喂给大鼠，可显著降低肝脏线粒体和微粒体的过氧化潜能。与从喂食对照或富含玉米油饮食的大鼠分离出的肝脏线粒体相比，来自喂食棕榈油组的线粒体对由抗坏血酸和NADPH诱导的过氧化表现出明显更低的敏感性。然而，在微粒体中，仅喂食富含棕榈油饮食的大鼠中由NADPH诱导的脂质过氧化显著降低。尽管在喂食补充了富含生育三烯酚组分（TRF）的棕榈油的富含玉米油饮食的大鼠的线粒体中，抗坏血酸诱导的脂质过氧化过程中硫代巴比妥酸反应性物质的积累与对照大鼠相似，但过氧化的初始速率比来自对照或喂食玉米油饮食的大鼠慢得多。我们的体外研究以及与过氧化潜能相关的辅助因子分析表明，在喂食棕榈油的大鼠中观察到的降低可能是由于生育三烯酚形式的抗氧化剂含量增加以及过氧化底物的可用性降低。
