Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, Hunan Key Laboratory of Processed Food for Special Medical Purpose, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha 410004, Hunan, China.
Department of Clinic Medicine, Xiangya School of Medicine, Central South University, Changsha 410008, Hunan, China.
Int J Mol Sci. 2018 Oct 4;19(10):3022. doi: 10.3390/ijms19103022.
δ-Tocotrienol, an important component of vitamin E, has been reported to possess some physiological functions, such as anticancer and anti-inflammation, however their molecular mechanisms are not clear. In this study, δ-tocotrienol was isolated and purified from rice bran. The anti-inflammatory effect and mechanism of δ-tocotrienol against lipopolysaccharides (LPS) activated pro-inflammatory mediator expressions in RAW264.7 cells were investigated. Results showed that δ-tocotrienol significantly inhibited LPS-stimulated nitric oxide (NO) and proinflammatory cytokine (TNF-α, IFN-γ, IL-1β and IL-6) production and blocked the phosphorylation of c-Jun N-terminal kinase (JNK) and extracellular regulated protein kinases 1/2 (ERK1/2). δ-Tocotrienol repressed the transcriptional activations and translocations of nuclear factor-kappa B (NF-κB) and activator protein-1 (AP-1), which were closely related with downregulated cytokine expressions. Meanwhile, δ-tocotrienol also affected the PPAR signal pathway and exerted an anti-inflammatory effect. Taken together, our data showed that δ-tocotrienol inhibited inflammation via mitogen-activated protein kinase (MAPK) and peroxisome proliferator-activated receptor (PPAR) signalings in LPS-stimulated macrophages.
δ-生育三烯酚是维生素 E 的重要组成部分，据报道具有一些生理功能，如抗癌和抗炎作用，但它们的分子机制尚不清楚。在本研究中，从米糠中分离和纯化了 δ-生育三烯酚。研究了 δ-生育三烯酚对脂多糖（LPS）激活的 RAW264.7 细胞中促炎介质表达的抗炎作用及其机制。结果表明，δ-生育三烯酚能显著抑制 LPS 刺激的一氧化氮（NO）和促炎细胞因子（TNF-α、IFN-γ、IL-1β和 IL-6）的产生，并阻断 c-Jun N 末端激酶（JNK）和细胞外调节蛋白激酶 1/2（ERK1/2）的磷酸化。δ-生育三烯酚抑制核因子-κB（NF-κB）和激活蛋白-1（AP-1）的转录激活和转位，这与细胞因子表达下调密切相关。同时，δ-生育三烯酚还影响过氧化物酶体增殖物激活受体（PPAR）信号通路，发挥抗炎作用。综上所述，我们的数据表明，δ-生育三烯酚通过丝裂原活化蛋白激酶（MAPK）和过氧化物酶体增殖物激活受体（PPAR）信号通路抑制 LPS 刺激的巨噬细胞中的炎症反应。
