用超热中子活化分析法简化蛋白质结合碘的测量。

Simplified measurement of protein-bound iodine with epithermal neutron activation analysis.

作者信息

Chou F I, Lin H D, Wei J C, Wang A Y, Lo J G

机构信息

Nuclear Science and Technology Development Center, National Tsing Hua University, Taiwan, Republic of China.

出版信息

Nucl Med Biol. 1993 Jul;20(5):631-6. doi: 10.1016/0969-8051(93)90032-p.

DOI:10.1016/0969-8051(93)90032-p

PMID:8358349

Abstract

A refined method for the measurement of protein-bound iodine (PBI) in blood serum was demonstrated by the use of epithermal neutron activation analysis (ENAA). PBI in ammonium sulfate-precipitated serum protein, after epithermal neutron activation, was determined by high resolution gamma-ray spectrometry. From our results, the PBI concentration was 67.8 +/- 2.2 ng/mL. Good agreement was obtained with published data, ranging from 40 to 80 ng/mL, which had been obtained using different analytical techniques. The validity of these techniques for PBI has been born out by a very good accuracy and simplicity without temperature dependence.

摘要

通过超热中子活化分析（ENAA）展示了一种用于测量血清中蛋白结合碘（PBI）的改进方法。超热中子活化后，通过高分辨率γ射线光谱法测定硫酸铵沉淀血清蛋白中的PBI。根据我们的结果，PBI浓度为67.8±2.2 ng/mL。与使用不同分析技术获得的已发表数据（范围为40至80 ng/mL）取得了良好的一致性。这些用于PBI的技术的有效性已通过非常高的准确性和简单性得到证实，且不受温度影响。