Koshiishi I, Motoki K, Qiu G, Imanari T
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Biol Pharm Bull. 1993 Apr;16(4):335-9. doi: 10.1248/bpb.16.335.
Dermatan sulfate-proteoglycans (DS-PGs) were extracted from rabbit, rat and bovine defatted livers by magnesium chloride extraction and DEAE-cellulose chromatography, and then submitted successively to Asahipak GS-520 gel filtration chromatography, Asahipak ES-502N anion exchange chromatography, and cellulose acetate membrane electrophoresis. The disaccharide composition of the glycosaminoglycan chains was determined by differential digestion by chondroitinase ABC, AC, ACII and/or B followed by HPLC for analysis of the resulting unsaturated disaccharides. The hepatic dermatan sulfate chains contained disulfated disaccharide units; Di-diSB and Di-diSE. The hepatic DS-PGs were divided into two groups; Di-diSE-poor DS-PGs and Di-diSE-rich DS-PGs. The iduronic acid content of Di-diSE-poor dermatan sulfate chains was higher than that of Di-diSE-rich ones.
通过氯化镁提取和DEAE-纤维素色谱法从兔、大鼠和牛的脱脂肝脏中提取硫酸皮肤素蛋白聚糖（DS-PGs），然后依次进行Asahipak GS-520凝胶过滤色谱法、Asahipak ES-502N阴离子交换色谱法和醋酸纤维素膜电泳。通过软骨素酶ABC、AC、ACII和/或B的差异消化，然后用HPLC分析所得不饱和二糖，来确定糖胺聚糖链的二糖组成。肝脏硫酸皮肤素链含有双硫酸化二糖单元；Di-diSB和Di-diSE。肝脏DS-PGs分为两组；低Di-diSE的DS-PGs和高Di-diSE的DS-PGs。低Di-diSE的硫酸皮肤素链的艾杜糖醛酸含量高于高Di-diSE的硫酸皮肤素链。
