大鼠腹膜常驻巨噬细胞中硫酸软骨素/硫酸皮肤素链的分析
Analysis of chondroitin sulfate/dermatan sulfate chains in rat peritoneal resident macrophages.
作者信息
Koshiishi I, Hayashi S, Imanari T
机构信息
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
出版信息
Biol Pharm Bull. 1993 Mar;16(3):307-8. doi: 10.1248/bpb.16.307.
PMID:8364478
Abstract
Chondroitin sulfate/dermatan sulfate (CS/DS) hybrid polymers obtained from rat peritoneal resident macrophages were analyzed by the enzymatic method; CS/DS chains were digested by chondroitinase, and the resultant unsaturated disaccharides were determined by HPLC with fluorometric detection. CS/DS in the resident macrophages are constituted of Di-4S, Di-6S, Di-diSE, Di-diSB and Di-triS.
摘要
采用酶法分析从大鼠腹膜常驻巨噬细胞获得的硫酸软骨素/硫酸皮肤素（CS/DS）杂合聚合物；用软骨素酶消化CS/DS链，通过高效液相色谱荧光检测法测定产生的不饱和二糖。常驻巨噬细胞中的CS/DS由二硫酸软骨素（Di-4S）、六硫酸软骨素（Di-6S）、二硫酸皮肤素（Di-diSE）、二硫酸皮肤素B（Di-diSB）和三硫酸软骨素（Di-triS）组成。
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