正常人尿液中硫酸皮肤素的分离与鉴定

Separation and characterization of dermatan sulfate in normal human urine.

作者信息

Qiu G, Tanikawa M, Akiyama H, Toida T, Koshiishi I, Imanari T

机构信息

Faculty of Pharmaceutical Sciences, Chiba University, Japan.

出版信息

Biol Pharm Bull. 1993 Apr;16(4):340-2. doi: 10.1248/bpb.16.340.

DOI:10.1248/bpb.16.340

PMID:8358381

Abstract

Dermatan sulfate excreted in normal human urine was isolated and characterized by TLC and cellulose acetate strip electrophoresis after cetylpyridinium chloride precipitation and pronase digestion. In these separation methods, dermatan sulfate and chondroitin sulfate were extracted and then monitored by sensitive HPLC methods with post column fluorometric derivatization coupled with chondroitinase ABC, ACII and B digestion. From the results, we demonstrated that human urinary dermatan sulfate contains iduronic acid as its major uronic acid (80-90% of total uronic acid), and is composed mainly of repeated mono-sulfated disaccharide units [Di-4S (structure shown in Fig. 1), 89%] and small numbers of di-sulfated disaccharide units (Di-diSB, 7% and Di-diSE, 1%).

摘要

通过十六烷基吡啶氯化物沉淀和链霉蛋白酶消化后，采用薄层层析（TLC）和醋酸纤维素条电泳对正常人尿液中排泄的硫酸皮肤素进行分离和表征。在这些分离方法中，提取硫酸皮肤素和硫酸软骨素，然后通过柱后荧光衍生结合软骨素酶ABC、ACII和B消化的灵敏高效液相色谱（HPLC）方法进行监测。结果表明，人尿硫酸皮肤素以艾杜糖醛酸作为其主要糖醛酸（占总糖醛酸的80 - 90%），主要由重复的单硫酸化二糖单元[Di - 4S（结构见图1），89%]和少量的双硫酸化二糖单元（Di - diSB，7%和Di - diSE，1%）组成。