Effect of 2-mercaptoethanol on the in vitro response of sensitized bovine peripheral blood lymphocytes to Anaplasma antigen.

Sensitized lymphocytes from 3 cows vaccinated with anaplasma antigen were isolated and caused to react in vitro with antigen in a microculture lymphocyte transformation test. The addition of 2-mercaptoethanol at a concentration of 25 mul of 4 X 10(-3) M solution/well significantly (P less than or equal to 0.01) increased [3H]thymidine incorporation compared to microculture without 2-mercaptoethanol.