Miller K G, Wendland B, Scheller R H
Howard Hughes Medical Institute, Beckman Center for Molecular and Genetic Medicine, Department of Molecular and Cellular Physiology, Stanford University Medical Center, CA 94305-5428.
Brain Res. 1993 Jul 9;616(1-2):99-104. doi: 10.1016/0006-8993(93)90197-u.
In this study, we used synaptic vesicles purified from the electric organ of marine electric rays to search for novel molecules which have important functions in synaptic transmission. Proteins that copurified with synaptic vesicles were used to immunize rats, and the resulting antisera were then used to further characterize the vesicle proteins. One of the antisera recognizes a protein of 34 kDa, p34, that has several characteristics which suggest it is a synaptic vesicle specific protein: (1) it copurifies exclusively with the synaptic vesicle peak during permeation chromatography on a controlled pore glass beads column, (2) it can be immunoprecipitated with intact synaptic vesicles and (3) it is specifically localized to the nervous system. The results suggest that p34 is a synaptic vesicle specific protein with a widespread distribution in the nervous system.
在本研究中，我们使用从海产电鳐的电器官中纯化得到的突触小泡来寻找在突触传递中具有重要功能的新分子。与突触小泡共同纯化的蛋白质被用来免疫大鼠，然后所得抗血清被用于进一步鉴定小泡蛋白。其中一种抗血清识别一种34 kDa的蛋白质，即p34，它具有几个表明其是突触小泡特异性蛋白质的特征：（1）在可控孔径玻璃珠柱上进行渗透色谱时，它仅与突触小泡峰共同纯化；（2）它可以被完整的突触小泡免疫沉淀；（3）它特异性定位于神经系统。结果表明p34是一种在神经系统中广泛分布的突触小泡特异性蛋白质。
