前列腺癌的基因分型：一项细胞遗传学、流式细胞术及原位DNA杂交的联合研究。

Genotypic characterization of prostatic carcinomas: a combined cytogenetic, flow cytometry, and in situ DNA hybridization study.

作者信息

Breitkreuz T, Romanakis K, Lutz S, Seitz G, Bonkhoff H, Unteregger G, Zwergel T, Zang K D, Wullich B

机构信息

Institute of Human Genetics, University of the Saar, Homburg/Saar, Germany.

出版信息

Cancer Res. 1993 Sep 1;53(17):4035-40.

PMID:8358732

Abstract

Cytogenetic studies were performed on 36 biopsies obtained from 26 primary prostatic adenocarcinomas. Following histopathological characterization of control sections, the biopsies were investigated using metaphase cytogenetics, DNA flow cytometry, and fluorescence in situ DNA hybridization. In 12 specimens, no carcinoma was found in control sections by histopathological means. In 24 carcinoma biopsies clonal aberrations were detected in 15 specimens. Tetraploidy as sole aberration was detected in five specimens. Loss of the Y chromosome was seen in eight samples. Only one tumor revealed structural abnormalities. Eight samples were found to be normal (46,XY). Remarkably, nonclonal chromosome aberrations, particularly marked chromosome loss, were frequently detected in prostatic carcinomas and premalignant lesions (prostatic intraepithelial neoplasia). In the series of biopsies investigated by means of cytogenetics and flow cytometry, biopsies with aneuploid DNA content were found to be cytogenetically normal. Conversely, the cytogenetically aberrant clones were found to be of diploid DNA content. Evidence of focal intratumoral heterogeneity was revealed by cytogenetics, flow cytometry, and in situ hybridization.

摘要

对取自26例原发性前列腺腺癌的36份活检样本进行了细胞遗传学研究。在对对照切片进行组织病理学特征分析后，采用中期细胞遗传学、DNA流式细胞术和荧光原位DNA杂交技术对活检样本进行研究。12份样本经组织病理学检查，对照切片中未发现癌组织。在24份癌组织活检样本中，15份检测到克隆性异常。5份样本检测到仅四倍体作为唯一异常。8份样本出现Y染色体丢失。仅1例肿瘤显示结构异常。8份样本被判定为正常（46,XY）。值得注意的是，在前列腺癌和癌前病变（前列腺上皮内瘤变）中经常检测到非克隆性染色体异常，尤其是明显的染色体丢失。在通过细胞遗传学和流式细胞术研究的一系列活检样本中，发现DNA含量为非整倍体的活检样本细胞遗传学正常。相反，细胞遗传学异常的克隆其DNA含量为二倍体。细胞遗传学、流式细胞术和原位杂交揭示了肿瘤内局灶性异质性的证据。