酿酒酵母线粒体基因组tRNA区域的对称转录

Symmetrical transcription in the tRNA region of the mitochondrial genome of Saccharomyces cerevisiae.

作者信息

Grisanti P, Francisci S, Tataseo P, Palleschi C

机构信息

Department of Developmental and Cell Biology, University of Rome, La Sapienza, Italy.

出版信息

Curr Genet. 1993 Jul-Aug;24(1-2):122-5. doi: 10.1007/BF00324675.

DOI:10.1007/BF00324675

Abstract

The occurrence of discrete transcripts originating from the non-coding strand of the yeast mitochondrial genome is described. The region under investigation is localized in the large tRNA gene cluster between the LSU ribosomal RNA and OXI 1 genes. The transcripts originating from the non-coding strand were detected in a wild-type strain and in a rho- mutant. Their size range includes transcripts of about 2000 nucleotides able to accommodate more than one "anti-tRNA". In some cases their extremities can be mapped near highly-conserved nonanucleotides that could function as origins of transcription. The involvement of the tRNA-processing machinery in the cleavage of these transcripts is also hypothesized.

摘要

本文描述了源自酵母线粒体基因组非编码链的离散转录本的出现情况。所研究的区域位于大核糖体RNA基因和OXI 1基因之间的大tRNA基因簇中。在野生型菌株和rho-突变体中均检测到了源自非编码链的转录本。它们的大小范围包括约2000个核苷酸的转录本，这些转录本能够容纳不止一个“反tRNA”。在某些情况下，它们的末端可以定位在可能作为转录起始点的高度保守的九核苷酸附近。还推测了tRNA加工机制参与这些转录本的切割过程。

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本文引用的文献

1

Sequence and organization of the human mitochondrial genome.

Nature. 1981 Apr 9;290(5806):457-65. doi: 10.1038/290457a0.

2

Assembly of the mitochondrial membrane system. Analysis of the nucleotide sequence and transcripts in the oxi1 region of yeast mitochondrial DNA.

J Biol Chem. 1981 Dec 25;256(24):12780-7.

3

Transcripts of mitochondrial tRNA genes in Saccharomyces cerevisiae.

Nucleic Acids Res. 1982 Nov 25;10(22):7283-93. doi: 10.1093/nar/10.22.7283.

4

A novel species of double stranded RNA in mitochondria of Saccharomyces cerevisiae.

Nucleic Acids Res. 1982 Feb 11;10(3):1051-70. doi: 10.1093/nar/10.3.1051.

5

Physical and genetic organization of petite and grande yeast mitochondrial DNA. IV. In vivo transcription products of mitochondrial DNA and localization of 23 S ribosomal RNA in petite mutants of saccharomyces cerevisiae.

J Mol Biol. 1974 Sep 5;88(1):185-203. doi: 10.1016/0022-2836(74)90304-0.

6

Two mammalian genes transcribed from opposite strands of the same DNA locus.

Science. 1987 Mar 20;235(4795):1514-7. doi: 10.1126/science.3547652.

7

Analysis of transcripts of the major cluster of tRNA genes in the mitochondrial genome of S. cerevisiae.

Nucleic Acids Res. 1987 Aug 25;15(16):6387-403. doi: 10.1093/nar/15.16.6387.

8

Gene within a gene: nested Drosophila genes encode unrelated proteins on opposite DNA strands.

Cell. 1986 Jan 17;44(1):33-42. doi: 10.1016/0092-8674(86)90482-4.

9

Structure of a precursor to the yeast mitochondrial tRNAMetf. Implications for the function of the tRNA synthesis locus.

J Biol Chem. 1985 Feb 10;260(3):1479-83.

10

An antisense transcript from the Xenopus laevis bFGF gene coding for an evolutionarily conserved 24 kd protein.

EMBO J. 1989 Oct;8(10):2983-8. doi: 10.1002/j.1460-2075.1989.tb08448.x.